Coding
Part:BBa_K1021001:Design
Designed by: Swati Sureka Group: iGEM13_Cornell (2013-08-30)
nptII (neomycin phosphotransferase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 796
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 629
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 478
Illegal SapI.rc site found at 688
Design Notes
The gene was extracted from its original vector, pNG, and inserted into pSB1C3. An illegal PstI site was then removed through site-directed mutagenesis.
Source
pNG, acquired from the lab of B. G. Turgeon and described in "ChLae1 and ChVel1 Regulate T-toxin Production, Virulence, Oxidative Stress Response, and Development of the Maize Pathogen Cochliobolus heterostrophus" (Wu et al. 2012).